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rabbit nf κb p65 polyclonal antibody hrp peroxidase conjugated bioss antibodies  (Bioss)
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Rabbit Nf κb P65 Polyclonal Antibody Hrp Peroxidase Conjugated Bioss Antibodies, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) We assessed T cell activation by measuring the expression of CD69 and CD25 on the cell surface. Purified CD4⁺ T cells from term and preterm neonates were either left unstimulated or stimulated under the indicated conditions for 72 hours. The percentages of CD69⁺ and CD25⁺ T cells were determined by flow cytometry. (B) For transcription factor activation, we evaluate phosphorylation of c-Jun and <t>p65</t> as markers of AP-1 and NF-κB activation, respectively. CBMCs were either left unstimulated or stimulated under the indicated conditions for 90 minutes (to measure c-Jun activation) or 60 minutes (for p65 activation). Mean fluorescence intensity (MFI) values for the indicated phosphoproteins were determined by flow cytometry. (C) MFI normalized values. The Wilcoxon test was used for paired samples, and the Mann–Whitney U test was used for unpaired samples. P values are shown above the error bars. * P < 0.05, ** P < 0.01. For the evaluation of CD69 and CD25 expression, the number of samples was as follows: vaginal delivery = 13, cesarean section = 9, and preterm birth = 3. For the evaluation of transcription factor activation, the sample sizes were: Natural birth = 7, Cesarean = 8, and Preterm = 5.
Anti Phospho P65, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) We assessed T cell activation by measuring the expression of CD69 and CD25 on the cell surface. Purified CD4⁺ T cells from term and preterm neonates were either left unstimulated or stimulated under the indicated conditions for 72 hours. The percentages of CD69⁺ and CD25⁺ T cells were determined by flow cytometry. (B) For transcription factor activation, we evaluate phosphorylation of c-Jun and <t>p65</t> as markers of AP-1 and NF-κB activation, respectively. CBMCs were either left unstimulated or stimulated under the indicated conditions for 90 minutes (to measure c-Jun activation) or 60 minutes (for p65 activation). Mean fluorescence intensity (MFI) values for the indicated phosphoproteins were determined by flow cytometry. (C) MFI normalized values. The Wilcoxon test was used for paired samples, and the Mann–Whitney U test was used for unpaired samples. P values are shown above the error bars. * P < 0.05, ** P < 0.01. For the evaluation of CD69 and CD25 expression, the number of samples was as follows: vaginal delivery = 13, cesarean section = 9, and preterm birth = 3. For the evaluation of transcription factor activation, the sample sizes were: Natural birth = 7, Cesarean = 8, and Preterm = 5.
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(A) We assessed T cell activation by measuring the expression of CD69 and CD25 on the cell surface. Purified CD4⁺ T cells from term and preterm neonates were either left unstimulated or stimulated under the indicated conditions for 72 hours. The percentages of CD69⁺ and CD25⁺ T cells were determined by flow cytometry. (B) For transcription factor activation, we evaluate phosphorylation of c-Jun and <t>p65</t> as markers of AP-1 and NF-κB activation, respectively. CBMCs were either left unstimulated or stimulated under the indicated conditions for 90 minutes (to measure c-Jun activation) or 60 minutes (for p65 activation). Mean fluorescence intensity (MFI) values for the indicated phosphoproteins were determined by flow cytometry. (C) MFI normalized values. The Wilcoxon test was used for paired samples, and the Mann–Whitney U test was used for unpaired samples. P values are shown above the error bars. * P < 0.05, ** P < 0.01. For the evaluation of CD69 and CD25 expression, the number of samples was as follows: vaginal delivery = 13, cesarean section = 9, and preterm birth = 3. For the evaluation of transcription factor activation, the sample sizes were: Natural birth = 7, Cesarean = 8, and Preterm = 5.
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(A) We assessed T cell activation by measuring the expression of CD69 and CD25 on the cell surface. Purified CD4⁺ T cells from term and preterm neonates were either left unstimulated or stimulated under the indicated conditions for 72 hours. The percentages of CD69⁺ and CD25⁺ T cells were determined by flow cytometry. (B) For transcription factor activation, we evaluate phosphorylation of c-Jun and <t>p65</t> as markers of AP-1 and NF-κB activation, respectively. CBMCs were either left unstimulated or stimulated under the indicated conditions for 90 minutes (to measure c-Jun activation) or 60 minutes (for p65 activation). Mean fluorescence intensity (MFI) values for the indicated phosphoproteins were determined by flow cytometry. (C) MFI normalized values. The Wilcoxon test was used for paired samples, and the Mann–Whitney U test was used for unpaired samples. P values are shown above the error bars. * P < 0.05, ** P < 0.01. For the evaluation of CD69 and CD25 expression, the number of samples was as follows: vaginal delivery = 13, cesarean section = 9, and preterm birth = 3. For the evaluation of transcription factor activation, the sample sizes were: Natural birth = 7, Cesarean = 8, and Preterm = 5.
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(A) We assessed T cell activation by measuring the expression of CD69 and CD25 on the cell surface. Purified CD4⁺ T cells from term and preterm neonates were either left unstimulated or stimulated under the indicated conditions for 72 hours. The percentages of CD69⁺ and CD25⁺ T cells were determined by flow cytometry. (B) For transcription factor activation, we evaluate phosphorylation of c-Jun and <t>p65</t> as markers of AP-1 and NF-κB activation, respectively. CBMCs were either left unstimulated or stimulated under the indicated conditions for 90 minutes (to measure c-Jun activation) or 60 minutes (for p65 activation). Mean fluorescence intensity (MFI) values for the indicated phosphoproteins were determined by flow cytometry. (C) MFI normalized values. The Wilcoxon test was used for paired samples, and the Mann–Whitney U test was used for unpaired samples. P values are shown above the error bars. * P < 0.05, ** P < 0.01. For the evaluation of CD69 and CD25 expression, the number of samples was as follows: vaginal delivery = 13, cesarean section = 9, and preterm birth = 3. For the evaluation of transcription factor activation, the sample sizes were: Natural birth = 7, Cesarean = 8, and Preterm = 5.
Anti P65, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) We assessed T cell activation by measuring the expression of CD69 and CD25 on the cell surface. Purified CD4⁺ T cells from term and preterm neonates were either left unstimulated or stimulated under the indicated conditions for 72 hours. The percentages of CD69⁺ and CD25⁺ T cells were determined by flow cytometry. (B) For transcription factor activation, we evaluate phosphorylation of c-Jun and <t>p65</t> as markers of AP-1 and NF-κB activation, respectively. CBMCs were either left unstimulated or stimulated under the indicated conditions for 90 minutes (to measure c-Jun activation) or 60 minutes (for p65 activation). Mean fluorescence intensity (MFI) values for the indicated phosphoproteins were determined by flow cytometry. (C) MFI normalized values. The Wilcoxon test was used for paired samples, and the Mann–Whitney U test was used for unpaired samples. P values are shown above the error bars. * P < 0.05, ** P < 0.01. For the evaluation of CD69 and CD25 expression, the number of samples was as follows: vaginal delivery = 13, cesarean section = 9, and preterm birth = 3. For the evaluation of transcription factor activation, the sample sizes were: Natural birth = 7, Cesarean = 8, and Preterm = 5.
Acetylated Nf κb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) We assessed T cell activation by measuring the expression of CD69 and CD25 on the cell surface. Purified CD4⁺ T cells from term and preterm neonates were either left unstimulated or stimulated under the indicated conditions for 72 hours. The percentages of CD69⁺ and CD25⁺ T cells were determined by flow cytometry. (B) For transcription factor activation, we evaluate phosphorylation of c-Jun and <t>p65</t> as markers of AP-1 and NF-κB activation, respectively. CBMCs were either left unstimulated or stimulated under the indicated conditions for 90 minutes (to measure c-Jun activation) or 60 minutes (for p65 activation). Mean fluorescence intensity (MFI) values for the indicated phosphoproteins were determined by flow cytometry. (C) MFI normalized values. The Wilcoxon test was used for paired samples, and the Mann–Whitney U test was used for unpaired samples. P values are shown above the error bars. * P < 0.05, ** P < 0.01. For the evaluation of CD69 and CD25 expression, the number of samples was as follows: vaginal delivery = 13, cesarean section = 9, and preterm birth = 3. For the evaluation of transcription factor activation, the sample sizes were: Natural birth = 7, Cesarean = 8, and Preterm = 5.
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(A) We assessed T cell activation by measuring the expression of CD69 and CD25 on the cell surface. Purified CD4⁺ T cells from term and preterm neonates were either left unstimulated or stimulated under the indicated conditions for 72 hours. The percentages of CD69⁺ and CD25⁺ T cells were determined by flow cytometry. (B) For transcription factor activation, we evaluate phosphorylation of c-Jun and <t>p65</t> as markers of AP-1 and NF-κB activation, respectively. CBMCs were either left unstimulated or stimulated under the indicated conditions for 90 minutes (to measure c-Jun activation) or 60 minutes (for p65 activation). Mean fluorescence intensity (MFI) values for the indicated phosphoproteins were determined by flow cytometry. (C) MFI normalized values. The Wilcoxon test was used for paired samples, and the Mann–Whitney U test was used for unpaired samples. P values are shown above the error bars. * P < 0.05, ** P < 0.01. For the evaluation of CD69 and CD25 expression, the number of samples was as follows: vaginal delivery = 13, cesarean section = 9, and preterm birth = 3. For the evaluation of transcription factor activation, the sample sizes were: Natural birth = 7, Cesarean = 8, and Preterm = 5.
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(A) We assessed T cell activation by measuring the expression of CD69 and CD25 on the cell surface. Purified CD4⁺ T cells from term and preterm neonates were either left unstimulated or stimulated under the indicated conditions for 72 hours. The percentages of CD69⁺ and CD25⁺ T cells were determined by flow cytometry. (B) For transcription factor activation, we evaluate phosphorylation of c-Jun and p65 as markers of AP-1 and NF-κB activation, respectively. CBMCs were either left unstimulated or stimulated under the indicated conditions for 90 minutes (to measure c-Jun activation) or 60 minutes (for p65 activation). Mean fluorescence intensity (MFI) values for the indicated phosphoproteins were determined by flow cytometry. (C) MFI normalized values. The Wilcoxon test was used for paired samples, and the Mann–Whitney U test was used for unpaired samples. P values are shown above the error bars. * P < 0.05, ** P < 0.01. For the evaluation of CD69 and CD25 expression, the number of samples was as follows: vaginal delivery = 13, cesarean section = 9, and preterm birth = 3. For the evaluation of transcription factor activation, the sample sizes were: Natural birth = 7, Cesarean = 8, and Preterm = 5.

Journal: bioRxiv

Article Title: PREMATURE BIRTH AND CESAREAN SECTION AFFECT NEONATAL CD4 + T CELL GENE EXPRESSION AND CELLULAR FUNCTION

doi: 10.64898/2026.02.21.707199

Figure Lengend Snippet: (A) We assessed T cell activation by measuring the expression of CD69 and CD25 on the cell surface. Purified CD4⁺ T cells from term and preterm neonates were either left unstimulated or stimulated under the indicated conditions for 72 hours. The percentages of CD69⁺ and CD25⁺ T cells were determined by flow cytometry. (B) For transcription factor activation, we evaluate phosphorylation of c-Jun and p65 as markers of AP-1 and NF-κB activation, respectively. CBMCs were either left unstimulated or stimulated under the indicated conditions for 90 minutes (to measure c-Jun activation) or 60 minutes (for p65 activation). Mean fluorescence intensity (MFI) values for the indicated phosphoproteins were determined by flow cytometry. (C) MFI normalized values. The Wilcoxon test was used for paired samples, and the Mann–Whitney U test was used for unpaired samples. P values are shown above the error bars. * P < 0.05, ** P < 0.01. For the evaluation of CD69 and CD25 expression, the number of samples was as follows: vaginal delivery = 13, cesarean section = 9, and preterm birth = 3. For the evaluation of transcription factor activation, the sample sizes were: Natural birth = 7, Cesarean = 8, and Preterm = 5.

Article Snippet: We used anti–phospho c-Jun (Ser63, cat. GTX61170, GeneTex), secondary anti–rabbit FITC-conjugated antibody (GeneTex), and anti–phospho p65 (Ser529)–APC (Miltenyi Biotec).

Techniques: Activation Assay, Expressing, Purification, Flow Cytometry, Phospho-proteomics, Fluorescence, MANN-WHITNEY